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US 10,947,563 B2Granted
Terpene synthases for biofuel production and methods thereof
AssigneeNational Technology & Engineering Solutions of Sandia, LLC
InventorsWeihua Wu, John Michael Gladden, Benjamin Chiau-Pin Wu, Ryan Wesley Davis
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Abstract
The present invention relates to terpene synthases capable of degrading precursors into biofuel compounds, such as terpenoid compounds. In one instance, a transformed organism can include such terpene synthases, as well as vectors encoding such synthases. Methods of employing such synthases and organisms are also described herein.
Claims20
A method of treating a biomass, the method comprising:
- exposing the biomass to one or more organisms of an isolated, genetically engineered organism, wherein the organism comprises:
- an exogenous terpenoid precursor, an exogenous enzyme configured to synthesize a terpenoid precursor, or a nucleic acid encoding the exogenous enzyme;
- and
- an exogenous terpene synthase or a nucleic acid encoding the exogenous terpene synthase, wherein the exogenous terpene synthase is selected from the group consisting of a pinene synthase, a guaiene synthase, a pinene and guaiene synthase, a caryophyllene synthase, a chamigrene synthase, a chamigrene and pinene synthase, a gurjunene synthase, a gurjunene and pinene synthase, a gumunene synthase, a selinene synthase, and an isoledene synthase, or a bifunctional synthase of any of these;
- and
- isolating one or more terpenoid compounds.
3 more elements
Dependencies are inferred from the claim text.