Methods for making and using modified oocytes
AssigneeNew York Stem Cell Foundation, Inc.
InventorDietrich M. Egli
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Abstract
The present invention provides modified oocytes having a nuclear genome derived from a first oocyte and cytoplasm derived from a second oocyte from a different subject, and methods for making and using such modified oocytes. The methods and compositions of the present invention can be useful in a variety of settings including, but not limited to, in in vitro fertilization (“IVF”) procedures.
Claims20
A method of treating infertility in a subject in need thereof, the method comprising using a modified oocyte in an assisted reproduction procedure, wherein the modified oocyte is produced by a method comprising:
- introducing a nuclear genome from a first oocyte that is developmentally incompetent and from a subject that is infertile, into an enucleated second oocyte that is developmentally competent to form the modified oocyte, wherein the nuclear genome is from a metaphase I oocyte or an immature metaphase II oocyte, and wherein the first oocyte and the enucleated second oocyte are mammalian and from mammals of the same species, thereby treating infertility in a subject in need thereof.
A method of treating infertility in a subject in need thereof, the method comprising using a modified oocyte in an assisted reproduction procedure, wherein the modified oocyte is produced by a method comprising:
- a) treating a karyoplast comprising a nuclear genome from a first oocyte that is developmentally incompetent and from a subject that is infertile to induce partial spindle depolymerization, wherein the nuclear genome is a mature metaphase II nuclear genome;
- and
- b) introducing the karyoplast comprising the partially depolymerized spindle into an enucleated second oocyte that is developmentally competent, thereby producing the modified oocyte, wherein the first oocyte and the second enucleated oocyte are mammalian and from mammals of the same species, thereby treating infertility in the subject.
Dependencies are inferred from the claim text.