Method of increasing the function of an AAV vector
AssigneeThe Trustees of the University of Pennsylvania
InventorsLuk Vandenberghe, Guangping Gao, James M. Wilson
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Abstract
A method of correcting singletons in a selected AAV sequence in order to increasing the packaging yield, transduction efficiency, and/or gene transfer efficiency of the selected AAV is provided. This method involves altering one or more singletons in the parental AAV capsid to conform the singleton to the amino acid in the corresponding position(s) of the aligned functional AAV capsid sequences.
Claims11
An adeno-associated virus (AAV) vector comprising an AAVrh32/33 capsid which has the amino acid sequence of SEQ ID NO:
- 2, wherein the viral vector carries a transgene encoding a gene product under the control of regulatory sequences which direct expression of the product in a host cell.
A nucleic acid molecule comprising a nucleic acid sequence encoding an AAV rh32/33 capsid, which has the amino acid sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.