ToLCNDV resistant melon plants

A method for producing a ToLCNDV resistant melon plant, comprising:

• a) selecting a ToLCNDV resistant donor plant,
• b) crossing the donor plant selected in a) with a plant sensitive to ToLCNDV,
• c) obtaining seeds from the plants crossed in b),
• wherein the seeds comprise an introgression fragment from chromosome 5 of a ToLCNDV resistant donor plant, wherein the introgression fragment confers ToLCNDV resistance and comprises a Thymine at nucleotide 68 of SEQ ID NO:
• 3 and/or an Adenine at nucleotide 227 of SEQ ID NO:
• 4, and wherein the introgression fragment is obtainable from seeds of which a representative sample has been deposited under Accession Number NCIMB 42585.

A method for producing melon seeds, comprising:

• a) growing a melon plant comprising at least one chromosome 5 having an introgression fragment from chromosome 5 of a ToLCNDV resistant donor plant, wherein the introgression fragment confers ToLCNDV resistance and comprises a Thymine at nucleotide 68 of SEQ ID NO:
• 3 and/or an Adenine at nucleotide 227 of SEQ ID NO:
• 4, and wherein the introgression fragment is obtainable from seeds of which a representative sample has been deposited under Accession Number NCIMB 42585,
• b) harvesting the fruits of the melon plants grown in a), and optionally
• c) collecting the seeds from the fruits obtained in b).

A method for producing hybrid melon seeds, comprising:

• a) crossing a first inbred melon plant comprising at least one chromosome 5 having an introgression fragment from chromosome 5 of a ToLCNDV resistant donor plant, wherein the introgression fragment confers ToLCNDV resistance and comprises a Thymine at nucleotide 68 of SEQ ID NO:
• 3 and/or an Adenine at nucleotide 227 of SEQ ID NO:
• 4 with a second inbred melon plant with or without a chromosome 5 having said introgression fragment, and wherein the introgression fragment is obtainable from seeds of which a representative sample has been deposited under Accession Number NCIMB 42585, and
• b) selecting seeds obtained from the cross of a).

A method for producing a melon fruit, comprising:

• a) growing a plant comprising at least one chromosome 5 having an introgression fragment from chromosome 5 of a ToLCNDV resistant donor plant, wherein the introgression fragment confers ToLCNDV resistance and comprises a Thymine at nucleotide 68 of SEQ ID NO:
• 3 and/or an Adenine at nucleotide 227 of SEQ ID NO:
• 4, and wherein the introgression fragment is obtainable from seeds of which a representative sample has been deposited under Accession Number NCIMB 42585, and
• b) harvesting the fruits produced by the plants grown in a).

A method of screening a plant or plant material or DNA derived therefrom for the presence of a fragment on chromosome 5 conferring TolCNDV resistance comprising screening genomic DNA from said plant or plant material for the SNP genotype of one or more of SNP 01 at nucleotide 101 of SEQ ID NO:

• 1, SNP 02 at nucleotide 945 of SEQ ID NO:
• 2, SNP 03 at nucleotide 68 of SEQ ID NO:
• 3, SNP 04 at nucleotide 227 of SEQ ID NO:
• 4, SNP 05 at nucleotide 839 of SEQ ID NO:
• 5 and SNP 06 at nucleotide 445 of SEQ ID NO:
• 6 and optionally selecting a plant or plant material that comprises the resistant donor genotype of one or more of SNP 01, SNP 02, SNP 03, SNP 04, SNP 05 and SNP 06 wherein the resistant donor genotype comprises a Cytosine for SNP 01 at nucleotide 101 of SEQ ID NO:
• 1, a Thymine for SNP 02 at nucleotide 945 of SEQ ID NO:
• 2, a Thymine for SNP 03 at nucleotide 68 of SEQ ID NO:
• 3, an Adenine for SNP 04 at nucleotide 227 of SEQ ID NO:
• 4, a Cytosine for SNP 05 at nucleotide 839 of SEQ ID NO:
• 5, and an Adenine for SNP 06 at nucleotide 445 of SEQ ID NO:
• 6, wherein the introgression fragment is obtainable from seeds of which a representative sample has been deposited under Accession Number NCIMB 42585.

A method for identifying or detecting a cultivated Cucumis melo plant comprising an introgression fragment on chromosome 5, wherein said introgression fragment comprises a ToLCNDV-resistance allele, comprising screening a Cucumis melo plant using a molecular marker assay that detects at least one of SNP marker of SNP 01 at nucleotide 101 in SEQ ID NO:

• 1, SNP 02 at nucleotide 945 in SEQ ID NO:
• 2, SNP 03 at nucleotide 68 in SEQ ID NO:
• 3, SNP 04 at nucleotide 227 in SEQ ID NO:
• 4, SNP 05 at nucleotide 839 in SEQ ID NO:
• 5 and/or SNP 06 at nucleotide 445 in SEQ ID NO:
• 6, and identifying and/or selecting a plant comprising the CC or CT genotype for SNP 01 in SEQ ID NO:
• 1, and/or the TT or TG genotype for SNP 02 in SEQ ID NO:
• 2, and/or the TT or TC genotype for the SNP 03 in SEQ ID NO:
• 3 and/or the AA or AG genotype for the SNP 04 in SEQ ID NO:
• 4, and/or the CC or CT genotype for SNP 05 in SEQ ID NO:
• 5, and/or the AA or AG genotype for SNP 06 in SEQ ID NO:
• 6, wherein the introgression fragment is obtainable from seeds of which a representative sample has been deposited under Accession Number NCIMB 42585.