US 10,947,556 B2 · Sequences to facilitate incorporation of DNA into the genome of an organism

Abstract

Methods and compositions are provided which allow for genetic modification of host cells including, plants and plant cells. The various methods and composition employ a recombinant DNA construct comprising SEQ ID NO: 1 and/or 2 or active variants and fragments thereof. Such polynucleotides find use in facilitating integration of polynucleotides of interest into the DNA of a host cell, including a plant or plant cell. Vectors, host cells, bacterium and plants comprising the recombinant DNA construct or fragments thereof are provided. Further provided are methods of introducing into a host cell or a plant cell a polynucleotide of interest. The method comprises contacting the host cell with a bacterium competent for the transformation of the host cell, wherein the bacterium comprises a transformation vector comprising a recombinant DNA construct.

Claims23

Dependent claims

A recombinant DNA construct comprising a polynucleotide of interest

(a) operably linked to SEQ ID NO:
1 or an active variant thereof comprising a sequence having at least 93% sequence identity to SEQ ID NO:
1;
and
(b) operably linked to SEQ ID NO:
2 or an active variant thereof comprising a sequence having at least 93% sequence identity to SEQ ID NO:
2;
wherein said active variant or variants facilitate integration of the polynucleotide of interest into DNA, and wherein SEQ ID NO:
1 or the active variant thereof and SEQ ID NO:
2 or the active variant thereof flank the polynucleotide of interest.

7 more elements

Dependencies are inferred from the claim text.

Sequences to facilitate incorporation of DNA into the genome of an organism

Life of this patent

Sequences to facilitate incorporation of DNA into the genome of an organism

Life of this patent

Abstract

Claims23