AP2 domain transcription factor ODP2 (ovule development protein 2) and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsWilliam J Gordon-Kamm, Timothy G Helentjaris, Keith S Lowe, Bo Shen, Mitchell C Tarczynski, Peizhong Zheng
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Abstract
Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
Claims16
A method for producing haploid plant embryos comprising expressing in a pollen cell, an OVULE DEVELOPMENT PROTEIN 2 (ODP2) polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:
- 26;
- and
- (b) an amino acid sequence having at least 85% sequence identity to SEQ ID NO:
- 26, or 28, wherein said polypeptide comprises two APETALA2 (AP2) domains and has ODP2 activity rendering pollen cells embryogenic;
- and,
- thereby inducing embryogenesis in the pollen cells;
- culturing the pollen cells to produce haploid plant embryos;
- and
- selecting a haploid plant embryo.
A method of promoting embryogenesis in a microspore or anther culture, wherein said method comprises introducing into a plant or a plant cell an OVULE DEVELOPMENT PROTEIN 2 (ODP2) nucleotide sequence that encodes an ODP2 polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:
- 26;
- and
- (b) an amino acid sequence having at least 85% sequence identity to SEQ ID NO:
- 26, wherein said polypeptide comprises two APETALA2 (AP2) domains and has ODP2 activity rendering the microspore or anther cultures embryogenic;
- and, thereby inducing embryogenesis in the microspore or anther cultures;
- and selecting an embryogenic microspore or embryogenic anther culture.
Dependencies are inferred from the claim text.