Production of organic acids from Aspergillus cis-aconitic acid decarboxylase (cadA) deletion strains
AssigneeBattelle Memorial Institute
InventorsShuang Deng, Ziyu Dai, Jon K. Magnuson
Life of this patent
Estimating the term…
Abstract
This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.
Claims15
An isolated recombinant Aspergillus fungus capable of producing 3-hydroxypropionic acid (3-HP), comprising:
- a genetic inactivation of an endogenous cis-aconitic acid decarboxylase (cadA) gene,
- an exogenous nucleic acid molecule encoding aspartate 1-decarboxylase (panD),
- an exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT), and
- an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH).
Dependencies are inferred from the claim text.