Recombinant microorganism having enhanced 2,3-butanediol producing ability and method for producing 2,3-butanediol using the same

A recombinant microorganism having enhanced 2,3-butanediol producing ability and decreased lactate producing ability, decreased ethanol producing ability, decreased formic acid producing ability and decreased acetic acid producing ability as compared to a corresponding wild-type microorganism,

• wherein an ldhA gene encoding a lactate dehydrogenase and a pflB gene encoding a pyruvate formate lyase are deleted in the recombinant microorganism,
• wherein the recombinant microorganism is a recombinant Klebsiella oxytoca microorganism, and the corresponding wild-type microorganism is a wild-type Klebsiella oxytoca microorganism,
• wherein the recombinant microorganism has higher selectivity of 2,3-butanediol or higher 2,3-butanediol productivity than a recombinant Klebsiella oxytoca in which an ldhA gene and a gene encoding alcohol dehydrogenation enzyme which converts acetyl-CoA to ethanol are deleted,
• wherein the recombinant microorganism has a selectivity of 2,3-butanediol of 70% or more based on batch culture or fed-batch culture, wherein the selectivity of 2,3-butanediol is calculated as:
• selectivity of 2,3-butanediol (%)={Production amount (g) of 2,3-butanediol/(production amounts (g) of 2,3-butanediol,ethanol,acetoin,succinic acid,lactate,formate, and acetic acid)}×100, and
• wherein the 2,3-butanediol productivity is calculated as:
• 2,3-butanediol productivity (g/L/h):
• an amount of 2,3-butanediol in grams produced per unit time of hours and unit volume of liters.