Interferon production using short RNA duplexes
AssigneeYale University
InventorsAnna Marie Pyle, Andrew Kohlway, Dahai Luo, David Rawling, Akiko Iwasaki
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Abstract
The present invention provides a small hairpin nucleic acid molecule that is capable of stimulating interferon production. The nucleic acid molecule of the present invention has a double-stranded section of less than 19 base pairs and at least one blunt end. In certain embodiments, the molecule comprises a 5′ triphosphate or a 5′ diphosphate.
Claims21
A ribonucleic acid (RNA) molecule capable of inducing an interferon response,
- wherein the RNA molecule is a single chain molecule and forms a hairpin structure consisting of a double-stranded section, a loop, and a blunt end,
- wherein the number of base pairs in the double stranded section is an integer equal to or greater than 10 and less than 18,
- wherein the molecule comprises a 5′-diphosphate terminus group, and
- wherein the molecule is capable of inducing an interferon response in a vertebrate cell.
A pharmaceutical composition comprising a RNA molecule capable of inducing an interferon response and a pharmaceutically acceptable carrier,
- wherein the RNA molecule is a single chain molecule and forms a hairpin structure consisting of a double-stranded section, a loop, and a blunt end,
- wherein the number of base pairs in the double stranded section is an integer equal to or greater than 10 and less than 18,
- wherein the RNA molecule comprises a 5′-terminus group selected from the group consisting of a 5′-triphosphate and a 5′-diphosphate,
- wherein the molecule is capable of inducing an interferon response in a vertebrate cell.
A ribonucleic acid (RNA) molecule capable of inducing an interferon response,
- wherein the RNA molecule is a single chain molecule and forms a hairpin structure consisting of a double-stranded section, a loop, and a blunt end,
- wherein the number of base pairs in the double stranded section is equal to or greater than 10 and less than 18, and
- wherein the molecule comprises a 5′-triphosphate terminus group,
- wherein the molecule is capable of inducing an interferon response in a vertebrate cell.
Dependencies are inferred from the claim text.