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US 10,947,517 B2Granted
CRISPR/Cas fusion proteins and systems
AssigneeSigma-Aldrich Co.
InventorFuqiang Chen
Life of this patent
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Abstract
Engineered Cas9 systems are disclosed herein. For example, Cas9-marker fusion proteins are provided. Peptide linkers which facilitate fusion of heterologous proteins to CRISPR proteins in ways that preserve CRISPR functionality are also provided.
Claims8
A fusion protein comprising a Streptococcus pyogenes Cas9 protein;
- a marker protein;
- at least one first linker between the Cas9 protein and the marker protein, wherein the at least one first linker comprises SEQ ID NO:35;
- and a first heterologous domain that is a nuclear localization signal;
- wherein the fusion protein is a nuclease and cleaves both strands of a double-stranded sequence, is a nickase and cleaves one strand of a double-stranded sequence, or has no nuclease or nickase activity.
1 more element
Dependencies are inferred from the claim text.