Exonuclease deficient polymerases
AssigneeRoche Sequencing Solutions, Inc.
InventorsAruna Ayer, Arkadiusz Bibillo, Preethi Sarvabhowman, Dhruti Vasudev Dalal, Ilya Lederman, Eileen Thai
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Abstract
Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.
Claims20
A modified DNA polymerase, derived from a parental DNA polymerase polypeptide having a DNA polymerase activity comprising an amino acid sequence having at least 80% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
- 1, comprising a substitution at a position corresponding to a position selected from the group consisting of T45, H123, L125, W127, D128, V179, S211, Y212, I215, T216, E219, Q221, E239, D241, Y259, Q260, A292, S293, and S294 of SEQ ID NO:
- 2, wherein the modified polymerase is exonuclease deficient.
An exonuclease-deficient DNA polymerase having a strand displacement activity, wherein said exonuclease-deficient DNA polymerase comprises an amino acid sequence having at least 80% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
- 1 and comprises a substitution at a position corresponding to a position selected from the group consisting of T45, H123, L125, W127, D128, V179, S211, Y212, I215, T216, E219, Q221, E239, D241, Y259, Q260, A292, S293, and S294 of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.