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AssigneesResearch Institute At Nationwide Children's Hospital, University of South Florida
InventorsMark Edward Peeples, Michael Nan-hao Teng, Octavio Ramilo, Maria Asuncion Mejias, Emilio Flano
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Embodiments disclosed herein provide compositions, methods, and uses for respiratory syncytial viruses (RSV) and immunogenic compositions thereof. Certain embodiments provide RSV having a mutated NS1 protein, where the mutation causes the uncoupling of the NS1 protein's replication and type I interferon (IFN) antagonist functions. In some embodiments, this uncoupling can produce virions capable of inducing a strong, long-lasting innate immune response while maintaining its ability to replicate in vitro. Also provided are methods for amplifying RSV in host cells, wherein amplified RSV has mutated NS1 protein in which the protein's replication and IFN antagonistic functions are uncoupled. In certain embodiments, the amplified RSV having mutated NS1 protein is formulated into immunogenic compositions, including vaccines. Other embodiments provide methods for inducing an effective immune response against RSV infection in a subject.
A mutant respiratory syncytial virus (RSV) comprising a mutated nonstructural protein 1 (NS1) comprising an amino acid deletion in the first 20 amino acids of an amino-terminus of the NS1, the amino acid deletion in the first 20 amino acids of the amino-terminus of the NS1 consisting of a mutation selected from the group consisting of Δ6-15;
Dependencies are inferred from the claim text.