Culture medium for epithelial stem cells and organoids comprising the stem cells
AssigneeKoninklijke Nederlandse Akademie van Wetenschappen
InventorsToshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Abstract
The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising said epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of said culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in said culture medium.
Claims4
An in vitro method for obtaining a gastric organoid, the method comprising culturing gastric epithelial stem cells or isolated tissue fragments comprising said gastric epithelial stem cells in contact with an extracellular matrix for a length of time sufficient to be able to observe formation of a gastric organoid, in a medium comprising Noggin as BMP inhibitor, EGF and FGF10 as mitogenic growth factors, and any one of R-spondin 1 through R-spondin 4 as a Wnt agonist, and further comprising one or more supplements selected from B27, N2, and N-Acetylcystein, and obtaining from the culture medium a gastric organoid.
Dependencies are inferred from the claim text.