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US 10,947,509 B2Granted
Cell populations, methods of transdifferentiation and methods of use thereof
AssigneesOrgenesis Inc., Tel HaShomer Medical Research Infrastructure and Services Ltd.
InventorSarah Ferber
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Abstract
The present invention provides methods for the sequential and temporally-regulated administration of pancreatic transcription factors to induce non-pancreatic cells to transdifferentiate and mature along the pancreatic β-cell lineage. The present invention also provides methods for identifying, isolating and enriching transdifferentiation predisposed cells and methods for treating a degenerative pancreatic disorder such as diabetes.
Claims12
A method of isolating a population of cells that have an enriched capacity for transcription factor induced transdifferentiation:
- (a) providing a heterogeneous population of human cells;
- (b) introducing a nucleic acid construct comprising a glutamine synthetase response element (GSRE), or fragment thereof capable of activating glutamine synthetase transcription, operatively linked to a reporter protein;
- (c) identifying the cells expressing the reporter protein;
- and
- (d) isolating the cells expressing the reporter protein;
- thereby isolating a cell population with enriched transdifferentiation capacity.
3 more elements
Dependencies are inferred from the claim text.