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US 10,947,508 B2Granted
Culture medium for mesenchymal stem cells
AssigneesAjinomoto Co., Inc., Kyoto University
InventorsYonghui Jin, Ikue Harata, Sho Senda, Hiroki Ozawa, Tsuyoshi Kobayashi, Junya Toguchida, Makoto Ikeya
Life of this patent
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Abstract
Mesenchymal stem cells may be culture for a long period, without using any special apparatus, equipment and the like, in a medium in which seven kinds of nonessential amino acids of glycine, alanine, serine, proline, asparagine, aspartic acid, and glutamic acid are reduced.
Claims10
A culture medium comprising histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, arginine, cysteine, glutamine, tyrosine, glycine, alanine, serine, proline, asparagine, aspartic acid, and glutamic acid,
- wherein said culture medium comprises components constituting basal medium, which are other than amino acid,
- wherein the concentrations of glycine, alanine, serine, proline, asparagine, aspartic acid, and glutamic acid in the culture medium are less than 5 μM, less than 5 μM, less than 3 μM, less than 5 μM, less than 1 μM, less than 2 μM, and less than 3 μM, respectively,
- wherein the basal medium is selected from DMEM, IMDM (Iscove's Modified Dulbecco's Medium), GMEM (Glasglow's MEM), RPMI-1640, α-MEM, Ham's Medium F-12, Ham's Medium F-10, Ham's Medium F12K, Medium 199, ATCC-CRCM30, DM-160, DM-201, BME, Fischer, McCoy's 5A, Leibovitz's L-15, RITC80-7, MCDB105, MCDB107, MCDB131, MCDB153, MCDB201, NCTC109, NCTC135, Waymouth's MB752/1, CMRL-1066, Williams' medium E, Brinster's BMOC-3 Medium, and a mixed medium thereof.
Dependencies are inferred from the claim text.