Liquid delivery device and cell culture device using the same

A liquid delivery device comprising:

• a first container for a cell culture medium;
• a second container for a cell suspension;
• a third container for culturing cells;
• a fourth container into which a liquid waste after cell culture is discarded;
• a fifth container for a scaffold solution containing a cell scaffold material;
• a first liquid delivery tube for delivering the cell culture medium contained in the first container;
• a second liquid delivery tube for delivering the cell suspension contained in the second container;
• a third liquid delivery tube connected to the first liquid delivery tube and the second liquid delivery tube, the third liquid delivery tube delivering to the third container the cell culture medium and the cell suspension that are present in the first liquid delivery tube and the second liquid delivery tube, respectively;
• a first liquid delivery pump provided on the third liquid delivery tube, the first liquid delivery pump controlling liquid delivery through the third liquid delivery tube;
• a bi-directional fourth liquid delivery tube for discharging the cell culture medium contained in the third container and delivering the scaffold solution to the third container;
• a fifth liquid delivery tube connected to the bi-directional fourth liquid delivery tube, the fifth liquid delivery tube delivering the scaffold solution contained in the fifth container to the third container through the bi-directional fourth liquid delivery tube;
• a sixth liquid delivery tube connected to the bi-directional fourth liquid delivery tube, the sixth liquid delivery tube discharging the cell culture medium contained in the third container into the fourth container through the bi-directional fourth liquid delivery tube;
• a second liquid delivery pump provided on the bi-directional fourth liquid delivery tube, the second liquid delivery pump controlling liquid delivery of the scaffold solution contained in the fifth container to the third container, and the cell culture medium from the third container into the fourth container through the bi-directional fourth liquid delivery tube;
• and
• a controller coupled to the first liquid delivery pump and the second liquid delivery pump, the controller configured to operate the first and second liquid delivery pumps to:
• control the first liquid delivery pump to operate in a first direction and deliver the cell culture medium and the cell suspension that are present in the first liquid delivery tube and the second liquid delivery tube, respectively, through the third liquid delivery tube to the third container,
• control the second liquid delivery pump to operate in a second direction and deliver the scaffold solution contained in the fifth container to the third container via the bi-directional fourth liquid delivery tube, and
• control the second liquid delivery pump to operate in a third direction opposite the second direction and deliver the cell culture medium from the third container into the fourth container through the bi-directional fourth liquid delivery tube.