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US 10,947,281 B2Granted
Identification of channelrhodopsin-2 (ChR2) mutations and methods of use
AssigneeWayne State University
InventorZhuo-hua Pan
Life of this patent
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Abstract
The invention provides compositions and kits including at least one nucleic acid or polypeptide molecule encoding for a mutant ChR2 protein. Methods of the invention include administering a composition comprising a mutant ChR2 to a subject to preserve, improve, or restore phototransduction. Preferably, the compositions and methods of the invention are provided to a subject having impaired vision, thereby restoring vision to normal levels.
Claims7
An isolated polypeptide molecule comprising SEQ ID NO:
- 26 in which the amino acid at position 132 is cysteine (C), wherein the amino acid at position 159 is serine (S), and wherein, in a retinal cell, the polypeptide confers light sensitivity at a light flicker frequency between 5 Hz-15 Hz and a slower off rate of the channel when compared to a Wild Type (WT) polypeptide comprising SEQ ID NO:
- 26.
Dependencies are inferred from the claim text.