Methods of purifying proteins
AssigneeBristol-Myers Squibb Company
InventorsZhengjian Li, Xuankuo Xu, Chao Huang, Zhiqiang Chen
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Abstract
In some embodiments, the present invention provides a method of purifying a protein of interest with a reduced level of aggregation formation in cation exchange (CEX) chromatography, comprising: (a) providing a mixture comprising the protein of interest and one or more contaminants; (b) loading the mixture onto a CEX resin coupled with arginine; and (c) eluting the protein of interest from the resin, thereby purifying the protein of interest with a reduced level of aggregation formation in CEX chromatography.
Claims16
A method of purifying a protein of interest with a reduced level of aggregation formation in cation exchange (CEX) chromatography, comprising:
- (a) providing a mixture comprising the protein of interest and one or more contaminants;
- (b) loading the mixture onto a CEX resin coupled with arginine;
- and
- (c) eluting the protein of interest from the resin, thereby purifying the protein of interest with a reduced level of aggregation formation in CEX chromatography.
Dependencies are inferred from the claim text.