Alternating flow column chromatography apparatus and method of use

A method of alternating flow column chromatography in an alternating flow column chromatography apparatus comprising:

• a separation column including at least one loading port for loading of components for separation;
• a first purification column in fluid communication with one end of the separation column and a second purification column in fluid communication with another end of the separation column;
• at least one eluent input port;
• an eluate output port;
• and an alternating flow valve in fluid communication with the at least one eluent input port, the eluate output port, the first purification column and the second purification column including the steps of:
• loading the separation column with sorbent powder and components for separation;
• delivering eluent through the alternating flow valve and the purification columns to the separation column;
• eluting eluate of a desired component from the separation column;
• collecting the eluate of desired component;
• and
• periodically operating the alternating flow valve to reverse the direction of the flow of eluent through the purification columns and the separation column.