# US 10,946,055 B2 · Method of synthesizing custard apple peel nanoparticles

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- Number: US 10,946,055 B2
- Document ID: US10946055B2
- Status: Granted
- Kind: B2
- Application number: 16/696,018
- Filed: 2019-11-26
- Published: 2020-10-08
- Granted: 2021-03-16
- Earliest claimed: 2019-04-05
- Assignees: King Saud University
- Applicants: King Saud University
- Inventors: Hany M. Yehia; Hatem Salama Ali; Ebtesam Mohammed Al Olayan; Manal Fawzy Elkhadragy; Mohamed Fekry Mansour Serag Eldin; Manal Ahmed Awad
- Web page: https://patentgrep.com/patent/US10946055B2

## CPC

- A61K 9/5176: {Compounds of unknown constitution, e.g. material from plants or animals (oils, fats, waxes, shellac A61K9/5123)}
- A61K 36/185: Magnoliopsida (dicotyledons)
- A61K 9/51: Nanocapsules; {Nanoparticles; (nanotubes A61K9/0092; polymeric micelles A61K9/1075; polymersomes A61K9/1273; pure drug nanoparticles A61K9/14; drug nanoparticles with adsorbed surface modifiers A61K9/141; conjugates, e.g. between drug and non-active nanoparticles, A61K47/50; preparations for in vivo diagnosis A61K49/00; with radioactive substances A61K51/00)}
- A61K 2236/33: involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K 2236/51: Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
- B82Y 5/00: Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES

## Abstract

The custard apple peel nanoparticles may be manufactured by extracting custard apple peels in a solvent, spraying the custard apple peel extracts into boiling water under ultrasonic conditions to produce a first mixture, sonicating the mixture, stirring the mixture, and drying the mixture to obtain custard apple peel nanoparticles. In an embodiment, the custard apple peel may be peel of Annona reticulata. In an embodiment, the custard apple peel nanoparticles may have improved antibacterial or antioxidant properties.

## Claims

### Claim 1

A pharmaceutical composition comprising an effective amount of custard apple (Annona reticulata) peel nanoparticles in combination with a pharmaceutically-acceptable carrier, wherein the custard apple (Annona reticulata) nanoparticles are synthesized by:
(a) dissolving about 450 mg of custard apple peel (epicarp) powder in a solvent comprising ethanol under stirrer to produce a solution;
(b) spraying the solution dropwise into about 40 ml of boiling water at a flow rate of about 0.2 ml per minute in 5 minutes under ultrasonic conditions including an ultrasonic power of about 750 W and a frequency of 20 kHz to produce an aqueous solution;
(c) sonicating the aqueous solution for about 10 minutes;
(d) stirring the solution at 200-800 rpm at room temperature for 20 minutes; and
(e) drying the solution of step (d) to obtain the custard apple peel nanoparticles.

### Claim 2 (depends on 1)

The pharmaceutical composition of claim 1, wherein the custard apple peel nanoparticles have an average size of about 190 nm.