# US 10,945,451 B2 · Yeast containing silage inoculants for the enhancement of silage digestion and fermentation in the rumen

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- Number: US 10,945,451 B2
- Document ID: US10945451B2
- Status: Granted
- Kind: B2
- Application number: 15/713,396
- Filed: 2017-09-22
- Published: 2018-01-11
- Granted: 2021-03-16
- Earliest claimed: 2012-06-12
- Assignees: Pioneer Hi-Bred International, Inc.
- Applicants: Pioneer Hi-Bred International, Inc.
- Inventors: Fredric Owens; Brenda Smiley
- Web page: https://patentgrep.com/patent/US10945451B2

## CPC

- A23K 30/18: using microorganisms or enzymes
- A23K 10/16: Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions (A23K10/12 takes precedence)
- A23K 10/18: of live microorganisms
- A23K 30/00: Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K 50/10: for ruminants
- A23K: FODDER

## Abstract

The invention relates to compositions for use as silage inoculants comprising a yeast strain, one or more bacterial stains, and a suitable carrier. The invention also relates to methods of improving livestock animal silage and meat and milk performance of a livestock animal.

## Claims

### Claim 1

A method for treating pre-ensiled plant material, the method comprising adding thereto: a yeast strain wherein the yeast strain is any one of: Saccharomyces cerevisiae strain YE206, deposited as Patent Deposit No. NRRL Y-50734; S. cerevisiae strain YE1241, deposited as Patent Deposit No. NRRL Y-50735; or S. cerevisiae strain YE1496, deposited as Patent Deposit No. NRRL Y-50736, one or more bacterial stains, and a suitable carrier.

### Claim 2 (depends on 1)

The method of claim 1, wherein the bacterial strains comprise one or more of: Lactobacillus plantarum, L. buchneri, L. alimentarius, L. crispatus, L. paralimentarius, L. brevis, and Enterococcus facium and mixtures thereof.

### Claim 3 (depends on 2)

The method of claim 2 wherein the bacterial strain comprises or more of: LP286, LN4017, LN4637, LP329, LP7109 or LN5689 and mixtures thereof.

### Claim 4 (depends on 1)

The method of claim 1, wherein the composition contains from about 101 to about 1010 viable organisms of said yeast strain per gram of a pre-ensiled plant material.

### Claim 5 (depends on 4)

The method of claim 4, wherein the composition contains from about 103 to about 106 viable organisms of said yeast strain per gram of said pre-ensiled plant material.

### Claim 6 (depends on 1)

The method of claim 1, wherein the pre-ensiled plant material is selected from the group consisting of grasses, maize, alfalfa, wheat, legumes, sorghum, sunflower, barley, grains, and mixtures thereof.