# US 10,945,449 B2 · Animal feed compositions and uses thereof

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- Number: US 10,945,449 B2
- Document ID: US10945449B2
- Status: Granted
- Kind: B2
- Application number: 15/579,769
- Filed: 2016-07-04
- Published: 2019-10-03
- Granted: 2021-03-16
- Earliest claimed: 2015-07-02
- Assignees: Novozymes A/S
- Applicants: Novozymes A/S
- Inventors: Ye Liu; Kirk Matthew Schnorr; Lars Kiemer; Lars Kobberoee Skov; Dorthe Hoej Sandvang; Marianne Thorup Cohn; Ming Li
- Web page: https://patentgrep.com/patent/US10945449B2

## CPC

- A23K 20/189: Enzymes
- A23K 20/147: Polymeric derivatives, e.g. peptides or proteins
- A23K 20/174: Vitamins
- A23K 20/28: Silicates, e.g. perlites, zeolites or bentonites
- A23K 50/30: for swines
- A23K 50/60: for weanlings
- A23K 50/75: for poultry
- A23K 50/80: for aquatic animals, e.g. fish, crustaceans or molluscs
- A61K 38/47: acting on glycosyl compounds (3.2), e.g. cellulases, lactases
- C12N 9/2462: {Lysozyme (3.2.1.17)}
- C12Y 302/01017: Lysozyme (3.2.1.17)
- A23K: FODDER
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

The present invention provides animal feed or animal feed additives comprising one or more polypeptides having lysozyme activity. The invention also provides polypeptides having lysozyme activity, polynucleotides encoding the polypeptides as well as methods of producing and using the polypeptides.

## Claims

### Claim 1

A method of treatment of a Clostridium perfringens infection and/or necrotic enteritis in an animal, comprising the steps of administering a polypeptide having lysozyme activity to the animal, wherein the polypeptide has at least 80% sequence identity to SEQ ID NO: 257.

### Claim 2

A method of improving the performance of an animal, comprising administering a polypeptide having lysozyme activity to the animal, wherein the polypeptide has at least 80% sequence identity to SEQ ID NO: 257.

### Claim 3 (depends on 2)

The method of claim 2, wherein the polypeptide has at least 95% sequence identity to SEQ ID NO: 257.

### Claim 4 (depends on 2)

The method of claim 2, wherein the animal is a poultry and the improved performance is improved weight gain, feed conversion ratio or European Production Efficacy Factor.

### Claim 5 (depends on 2)

The method of claim 2, wherein the animal is a piglet and the improved performance is improved feed conversion ratio or body weight gain.

### Claim 6

A nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having lysozyme activity, wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in a recombinant host cell and wherein the polypeptide has at least 80% sequence identity to SEQ ID NO: 257.

### Claim 7 (depends on 6)

A recombinant host cell comprising the nucleic acid construct of claim 6.

### Claim 8 (depends on 7)

A method of producing a polypeptide having lysozyme activity, comprising:
(a) cultivating the recombinant host cell of claim 7 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

### Claim 9 (depends on 8)

The method of claim 8, wherein the polypeptide has at least 95% sequence identity to SEQ ID NO: 257.