# US 10,945,442 B2 · Mite composition, method for rearing a phytoseiid predatory mite species, and use of the composition for controlling crop pests

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- Number: US 10,945,442 B2
- Document ID: US10945442B2
- Status: Granted
- Kind: B2
- Application number: 15/858,081
- Filed: 2017-12-29
- Published: 2018-06-14
- Granted: 2021-03-16
- Earliest claimed: 2010-04-23
- Assignees: AgrobíO S.L.
- Applicants: Agrobio S.L.
- Inventors: Enrique Vila Rifà; Don Griffiths
- Web page: https://patentgrep.com/patent/US10945442B2

## CPC

- A01N 63/16: Arachnids
- A01K 67/30: Rearing or breeding invertebrates
- A23K 50/90: for insects, e.g. bees or silkworms
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A23K: FODDER

## Abstract

The present invention relates to a mite composition, the rearing thereof and to the use of the composition for controlling crop pests. The invention provides a mite composition comprising: —a rearing population of a phytoseiid predatory mite species, —a population of at least one species from the order Astigmata, and—optionally a carrier, wherein the population of the species from the order Astigmata is not alive. In particular the population of the species from the order Astigmata is in fast frozen fom1. The composition is used for controlling crop pests, such as thrip species. The crops may be greenhouse grown crops and open field crops.

## Claims

### Claim 1

A method for rearing a phytoseiid predatory mite comprising:
providing a mite composition comprising:
a rearing population of a phytoseiid predatory mite species, wherein the phytoseiid predatory species is from the subfamily Amblyseiinae;
a population of at least one species from the order Astigmata, wherein the at least one species from the order Astigmata is dead before and after being combined with the rearing population of the phytoseiid predatory mite species; and optionally a carrier,
allowing individuals of the phytoseiid predatory mite to prey on the population of the species from the order Astigmata.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the rearing population is maintained at 20-30° C. and 70-90% relative humidity.

### Claim 3 (depends on 2)

The method according to claim 2, wherein the relative humidity is 75-85%.

### Claim 4 (depends on 1)

The method according to claim 1, further comprising the step of fast freezing the population of the species from the order Astigmata.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the phytoseiid predatory species from the subfamily Amblyseiinae is selected from the group consisting of the genus Amblyseius, the genus Neoseiulus, the genus Typhlodromalus, the genus Typhlodromips, and the genus Euseius.

### Claim 6 (depends on 5)

The method according to claim 5, wherein the phytoseiid predatory species is selected from the group consisting of Amblyseius swirskii, Amblyseius cucumeris, Amblyseius largoensis, Amblyseius andersoni; Neoseiulus womersleyi, Neoseiulus californicus, Neoseiulus fallacis, Neoseiulus longispinosus; Typhlodromalus lailae, Typhlodromalus limonicus; Typhlodromips montdorensis; Euseius ovalis, Euseius scutalis, Euseius tularensis, and Euseius hibisci.

### Claim 7 (depends on 1)

The method according to claim 1, further comprising the step of selecting the at least one species from the order Astigmata from the members of the family Acaridae, from the family Carpoglyphidae, from the family Glycyphagidae, and/or from the family Chortoglyphagidae.

### Claim 8 (depends on 7)

The method according to claim 7, wherein the at least one species from the order Astigmata is selected from members of the genus Acarus, the genus Tyrophagus, the genus Aleuroglyphus, the genus Lardoglyphus, the genus Caloglyphus, the genus Suidasia, the genus Thyreophagus, the genus Carpoglyphus, the genus Glycyphagus, the genus Lepidoglyphus, the genus Blomia, and/or the genus Chortoglyphus.

### Claim 9 (depends on 7)

The method according to claim 7, wherein the at least one species from the order Astigmata is selected from the species: Acarus siro, Acarus farris, Acarus immobilis, Acarus chaetoxysilos, Tyrophagus longior, Tyrophagus similis, Tyrophagus putrescentiae, Aleuroglyphus ovatus, Lardoglyphus konoi, Caloglyphus mycophagus, Suidasia nesbitti, Thyreophagus entomophagus, Carpoglyphus lactis, Carpoglyphus munroi, Glycyphagus domesticus, Lepidoglyphus destructor, Blomia freemani and/or Chortoglyphus arcuatus.

### Claim 10 (depends on 1)

The method according to claim 1, further comprising the step of providing a further nutritional source for the phytoseiid population.

### Claim 11 (depends on 10)

The method according to claim 10, wherein the further nutritional source for the phytoseiid population comprises pollen.

### Claim 12 (depends on 1)

The method according to claim 1, further comprising reducing or removing the food material used to feed the prey mites.

### Claim 13 (depends on 1)

The method according to claim 1, further comprising reducing fungal contamination by exhaustion of the food material for the prey mites.

### Claim 14 (depends on 1)

The method according to claim 1, further comprising protecting the protein content of the prey mites from denaturing.