# US 10,945,439 B2 · Pseudomonas strains and consortia thereof for use in protection against plant diseases > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,945,439 B2 - Document ID: US10945439B2 - Status: Granted - Kind: B2 - Application number: 16/092,457 - Filed: 2017-04-12 - Published: 2019-04-25 - Granted: 2021-03-16 - Earliest claimed: 2016-04-12 - Assignees: Wim de Laat Consultancy B.V. - Applicants: Wim de Laat Consultancy B.V. - Inventors: Wilhelmus Theodorus Antonius Maria de Laat; Erwin Suir; Ap de Haan; Joyce Josephine Elisabeth Arnouts - Web page: https://patentgrep.com/patent/US10945439B2 ## CPC - C12N 1/20: Bacteria; Culture media therefor - A01N 63/27: Pseudomonas - C12N 1/205: Bacterial isolates - C12R 2001/38: Pseudomonas - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS ## Abstract The present invention relates Pseudomonas strains and consortia thereof that are useful in protecting plants against microbial plant diseases caused by pathogens such as Ralstonia, Clavibacter, Erwinia, Curtobacterium, Fusarium, Phytophthora and Helminthosporium. The Pseudomonas strains were selected on the basis of their antagonistic abilities against plant pathogens such as production of antimicrobial compounds, direct inhibition of growth of plant pathogens, competition of carbon or nitrogen sources and endophytic features such as anaerobic growth on nitrate as electron acceptor and growth on arabinose as carbon source. The invention further relates to compositions comprising the strains or consortia of the invention, preferably lyophilized compositions, and to methods wherein they are used in protecting a wide variety of plants against a wide variety of microbial plant diseases. ## Claims ### Claim 1 A consortium the consortium comprising at least 2, 3, 4, 5, 6, 7, or 8 strains selected from the strains 17, 55, Pr, Br, 27, 80, 20, 24, 11 and 29. ### Claim 2 (depends on 1) The consortium according to claim 1, wherein the consortium is comprised in one or more compositions, each composition comprising one or more of the strains of the consortium and an agriculturally acceptable carrier. ### Claim 3 (depends on 2) The consortium according to claim 2, wherein at least one of the compositions is a lyophilized composition. ### Claim 4 (depends on 2) The consortium according to claim 2, wherein each strain of the consortium is present at a concentration ranging from about 1×102 to about 5×1011 CFU per gram. ### Claim 5 (depends on 4) The consortium according to claim 4, wherein each strain of the consortium is present at a concentration that is higher than about 1×102 CFU per gram. ### Claim 6 (depends on 1) The consortium according to claim 1, wherein the consortium comprises at least 2, 3, 4, 5 or 6 strains selected from the strains 17, 55, Pr, Br, 11 and 29. ### Claim 7 (depends on 6) The consortium according to claim 6, wherein the consortium comprises the strains 17, 55, Pr, Br, 11 and 29. ### Claim 8 (depends on 7) The consortium according to claim 7, wherein the consortium consists of the strains 17, 55, Pr, Br, 11 and 29. ### Claim 9 (depends on 1) A method of protecting plants against a plant pathogen comprising applying to plants, plant tubers, plant seeds, plant roots or soil surrounding plants, plant tubers, plant seeds, plant roots or plant cuttings, a consortium according to claim 1, under conditions effective to protect said plants or the plants produced from said plant cuttings, tubers or seeds against the plant pathogen. ### Claim 10 (depends on 9) The method according to claim 9, wherein the plant pathogen is a selected from the Group consisting of Ralstonia, Clavibacter, Xanthomonas, Erwinia, Curtobacterium, Fusarium, Rhizoctonia, Verticillium, Pythium, Botrytis, Phytophthora and Helminthosporium. ### Claim 11 (depends on 9) The method according to claim 9, wherein the consortium is used to treat the plant by topical application, to treat soil around the plant's roots or wherein the consortium is applied to seed of the plant to be protected. ### Claim 12 (depends on 6) The consortium according to claim 6, wherein the consortium comprises at least 2 or 3 strains selected from the strains 17, 55, Pr and Br.