# US 10,945,437 B2 · Composition comprising bacillus

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- Number: US 10,945,437 B2
- Document ID: US10945437B2
- Status: Granted
- Kind: B2
- Application number: 15/346,621
- Filed: 2016-11-08
- Published: 2017-02-23
- Granted: 2021-03-16
- Earliest claimed: 2010-08-10
- Assignees: Chr. Hansen A/S
- Applicants: Chr. Hansen A/S
- Inventors: Abilio Alessandri; Inge Knap; Luciana Sekito De Freitas Zambelli
- Web page: https://patentgrep.com/patent/US10945437B2

## CPC

- C12N 1/20: Bacteria; Culture media therefor
- A01N 25/14: wettable
- A01N 63/22: Bacillus
- C12N 1/205: Bacterial isolates
- C12R 2001/10: Bacillus licheniformis
- C12R 2001/125: Bacillus subtilis {; Hay bacillus; Grass bacillus}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present invention refers to a composition comprising Bacillus subtilis (DSM 17231) and Bacillus licheniformis (DSM 17236) with nematicidal effect against phytonematodes on plants and/or its habitat, to its use and process for its preparation, use of Bacillus subtilis (DSM 17231) and Bacillus licheniformis (DSM 17236), processes for controlling, combating and conferring specific resistance to phytonematodes, and a kit.

## Claims

### Claim 1

A kit comprising
a composition comprising: a Bacillus subtilis strain DSM 17231 with retained or improved nematicidal properties, or a mutant thereof having all of the identifying characteristics thereof; a Bacillus licheniformis strain DSM 17236 with retained or improved nematicidal properties, or a mutant thereof having all of the identifying characteristics thereof; and at least one agrochemically acceptable excipient or carrier, wherein the composition is in the form of a wettable powder, a solution, a slurry, or granules, and wherein each of the strains, or mutants thereof, is present in an amount sufficient for controlling, combating and/or conferring specific resistance to phytonematodes; and
instructions for using the kit.

### Claim 2 (depends on 1)

The kit of claim 1, wherein the instructions for using the kit include instructions to treat at a dosing amount of at least about 4 kg-h−1.

### Claim 3 (depends on 1)

The kit of claim 1, wherein the instructions for using the kit include instructions to treat at a dosing amount of about 4 kg-h−1 to about 8 kg-h−1.

### Claim 4 (depends on 1)

The kit of claim 1, wherein the instructions for using the kit include instructions to apply the composition to plants being affected by phytonematodes selected from the group consisting of Meloidogyne incognita, Meloidogyne javanica, Meloidogyne exigua, and Meloidogyne paranaensis.

### Claim 5 (depends on 1)

The kit of claim 1, wherein the composition does not comprise a synthetic pesticide.