# US 10,945,427 B2 · Trehalose and dextran-containing solution for transplanting mammalian cells

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- Number: US 10,945,427 B2
- Document ID: US10945427B2
- Status: Granted
- Kind: B2
- Application number: 14/895,366
- Filed: 2014-06-18
- Published: 2016-05-05
- Granted: 2021-03-16
- Earliest claimed: 2013-06-28
- Assignees: Otsuka Pharmaceutical Factory, Inc.
- Applicants: Otsuka Pharmaceutical Factory, Inc.
- Inventors: Masuhiro Nishimura; Tamaki Wada; Chikage Shirakawa; Masako Doi
- Web page: https://patentgrep.com/patent/US10945427B2

## CPC

- A01N 1/126: Physiologically active agents, e.g. antioxidants or nutrients
- A01N 1/10: Preservation of living parts
- A01N 1/122: Preservation or perfusion media
- A01N 1/125: Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
- A61K 9/008: {comprising drug dissolved or suspended in liquid propellant for inhalation via a pressurized metered dose inhaler [MDI]}
- A61K 9/08: Solutions {(composition of solutions A61K47/00)}
- A61K 35/28: Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The problem of the present invention is to provide a method for preserving mammalian cells over a long period of time using a solution for cell transplantation, capable of effectively suppressing cell death when the mammalian cells have been preserved, and the solution for cell transplantation. The present invention is characterized in that mammalian cells are preserved in a physiological aqueous solution for cell transplantation, comprising 2.0 to 6.0% (w/v) of trehalose, a derivative thereof, or a salt of trehalose or the derivative (a trehalose) and 4.0 to 7.0% (w/v) of dextran, a derivative thereof, or a salt of dextran or the derivative (a dextran). The effects of a trehalose and a dextran contained in the physiological aqueous solution for cell transplantation can suppress a decrease in the cell survival rate when mammalian cells are preserved for a long period of time (at least 14 days).

## Claims

### Claim 1

A method for preserving a mammalian cell in a physiological aqueous solution for cell transplantation, the method comprising preserving the mammalian cell for 3 to 14 days in a physiological aqueous solution for cell transplantation, said physiological aqueous solution further comprising 2.0 to 6.0% w/v of trehalose, a derivative thereof, or a salt of trehalose or the derivative, and 4.0 to 7.0% w/v of dextran, a derivative thereof, or a salt of dextran or the derivative.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the physiological aqueous solution is selected from the group consisting of lactate Ringer's solution, saline, Ringer's solution, and acetate Ringer's solution.

### Claim 3 (depends on 2)

The method according to claim 2, wherein the mammalian cell is a mammalian mesenchymal stem cell or mammalian T cell.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the mammalian cell is a mammalian mesenchymal stem cell or mammalian T cell.

### Claim 5 (depends on 4)

The method according to claim 4, wherein the mammalian mesenchymal stem cell is a human mesenchymal stem cell from bone marrow and the mammalian T cell is a human peripheral blood T cell.

### Claim 6

A method for preserving a mammalian cell in a physiological aqueous solution for cell transplantation, the method comprising:
adding a mammalian cell to a physiological aqueous solution for cell transplantation, said physiological aqueous solution further comprising 2.0 to 6.0% w/v of trehalose, a derivative thereof, or a salt of trehalose or the derivative, and 4.0 to 7.0% w/v of dextran, a derivative thereof, or a salt of dextran or the derivative to preserve the mammalian cell for 3 to 14 days, or
adding trehalose, a derivative thereof, or a salt of trehalose or the derivative to 2.0 to 6.0% w/v and dextran, a derivative thereof, or a salt of dextran or the derivative to 4.0 to 7.0% w/v to a physiological aqueous solution containing a mammalian cell to preserve the mammalian cell for 3 to 14 days.

### Claim 7 (depends on 6)

The method according to claim 6, wherein the physiological aqueous solution is selected from the group consisting of lactate Ringer's solution, saline, Ringer's solution, and acetate Ringer's solution.

### Claim 8 (depends on 7)

The method according to claim 7, wherein the mammalian cell is a mammalian mesenchymal stem cell or mammalian T cell.

### Claim 9 (depends on 6)

The method according to claim 6, wherein the mammalian cell is a mammalian mesenchymal stem cell or mammalian T cell.

### Claim 10 (depends on 9)

The method according to claim 9, wherein the mammalian mesenchymal stem cell is a human mesenchymal stem cell from bone marrow and the mammalian T cell is a human peripheral blood T cell.