Genetically modified non-human animal with human or chimeric CD3e
AssigneesBiocytogenPharmaceuticals (Beijing) Co., Ltd., Biocytogen JiangSu Co., Ltd.
InventorsYuelei Shen, Yanan Guo, Rui Huang, Xiaofei Zhou, Yang Bai, Jiawei Yao, Chaoshe Guo
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Abstract
The present disclosure relates to genetically modified non-human animals that express a human or chimeric (e.g., humanized) CD3e (T-cell surface glycoprotein CD3 epsilon chain), and methods of use thereof.
Claims18
A genetically-modified, non-human mammal whose genome comprises a replacement of a nucleic acid sequence encoding a portion of the extracellular region of an endogenous CD3e with a nucleic acid sequence encoding the corresponding portion of the extracellular region of a human CD3e, wherein the replacement results in a sequence encoding a chimeric CD3e comprising an amino acid sequence that is at least 95% identical to amino adds 1-126 of SEQ ID NO:
- 7, at an endogenous CD3e gene locus, wherein the chimeric CD3e associates with endogenous CD3γ and endogenous CD3δ, forming a functional CD3 complex in the mammal, wherein the non-human mammal detectably expresses the chimeric CD3e on the surface of one or more activated T cells.
A genetically-modified, non-human mammal whose genome comprises a replacement of a nucleic acid sequence encoding the extracellular region of an endogenous CD3e with a nucleic acid sequence encoding the corresponding extracellular region of a human CD3e, wherein the replacement results in a sequence encoding a chimeric CD3e comprising an amino acid sequence that is at least 95% identical to amino acids 1-126 of SEQ ID NO:
- 7, at an endogenous CD3e gene locus, wherein the chimeric CD3e associates with endogenous CD3γ and endogenous CD3δ, forming a functional CD3 complex in the mammal, wherein the non-human mammal detectably expresses the chimeric CD3e on the surface of one or more activated T cells.
A genetically-modified, non-human mammal whose genome comprises a replacement of a nucleic acid sequence encoding an endogenous CD3e with a corresponding nucleic acid sequence encoding a human CD3e, wherein the replacement results in a sequence encoding a chimeric CD3e comprising an amino acid sequence that is at least 95% identical to amino acids 1-126 of SEQ ID NO:
- 7, at an endogenous CD3e gene locus, wherein the human CD3e associates with endogenous CD3γ and endogenous CD3 δ, forming a functional CD3 complex in the mammal, wherein the non-human mammal detectably expresses the human CD3e on the surface of one or more activated T cells.
Dependencies are inferred from the claim text.