# US 10,945,395 B2 · Lettuce variety Takita

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- Number: US 10,945,395 B2
- Document ID: US10945395B2
- Status: Granted
- Kind: B2
- Application number: 16/527,507
- Filed: 2019-07-31
- Published: 2020-07-09
- Granted: 2021-03-16
- Earliest claimed: 2019-07-31
- Assignees: Syngenta Crop Protection AG
- Applicants: Syngenta Crop Protection AG
- Inventors: Miguel Roca
- Web page: https://patentgrep.com/patent/US10945395B2

## CPC

- A01H 6/1472: {Lactuca sativa [lettuce]}
- A01H 5/12: Leaves
- C12N 15/8286: {for insect resistance}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention provides novel lettuce cultivar Takita and plant parts, seed, and tissue culture therefrom. The invention also provides methods for producing a lettuce plant by crossing the lettuce plants of the invention with themselves or another lettuce plant. The invention also provides lettuce plants produced from such a crossing as well as plant parts, seed, and tissue culture therefrom.

## Claims

### Claim 1

A seed of lettuce cultivar Takita, a representative sample of seed having been deposited under ATCC Accession No. PTA-126290.

### Claim 2

A plant of lettuce cultivar Takita, a representative sample of seed having been deposited under ATCC Accession No. PTA-126290.

### Claim 3 (depends on 2)

A lettuce plant, or a part thereof, having all of the physiological and morphological characteristics of the lettuce plant of claim 2.

### Claim 4 (depends on 2)

An F1 progeny lettuce plant of the plant of claim 2.

### Claim 5 (depends on 4)

A seed that produces the plant of claim 4.

### Claim 6 (depends on 2)

A plant part of the lettuce plant of claim 2.

### Claim 7 (depends on 6)

The plant part of claim 6, wherein the plant part is a leaf, pollen, an ovule, an anther, a root, or a cell.

### Claim 8 (depends on 2)

A tissue culture of regenerable cells of the plant of claim 2.

### Claim 9 (depends on 8)

A lettuce plant regenerated from the tissue culture of claim 8 or a selfed progeny thereof, wherein said lettuce plant expresses all of the physiological and morphological characteristics of lettuce cultivar Takita.

### Claim 10 (depends on 2)

A processed product from the plant of claim 2, wherein the processed product comprises cut, sliced, ground, pureed, dried, canned, jarred, washed, packaged, frozen and/or heated leaves.

### Claim 11 (depends on 2)

A converted lettuce plant produced by introducing a single locus conversion into the plant of claim 2, wherein said converted lettuce plant comprises said single locus conversion and otherwise comprises all of the physiological and morphological characteristics of lettuce cultivar Takita.

### Claim 12 (depends on 11)

A seed that produces the plant of claim 11.

### Claim 13 (depends on 2)

A method of producing lettuce seed, the method comprising crossing the plant of claim 2 with itself or a second lettuce plant and harvesting the resulting seed.

### Claim 14 (depends on 13)

An F1 lettuce seed produced by the method of claim 13.

### Claim 15 (depends on 14)

A lettuce plant, or part thereof, produced by growing the seed of claim 14.

### Claim 16 (depends on 2)

A method for producing a seed of a lettuce plant derived from the plant of claim 2, the method comprising:
(a) crossing a plant of lettuce cultivar Takita with a second lettuce plant; and
(b) allowing seed to form;
(c) growing a plant from the seed of step (b) to produce a plant derived from lettuce cultivar Takita;
(d) selfing the plant of step (c) or crossing it to a second lettuce plant to form additional lettuce seed derived from lettuce cultivar Takita; and
(e) optionally repeating steps (c) and (d) one or more times to generate further derived lettuce seed from lettuce cultivar Takita, wherein in step (c) a plant is grown from the additional lettuce seed of step (d) in place of growing a plant from the seed of step (b).

### Claim 17 (depends on 2)

A method of vegetatively propagating the plant of claim 2, the method comprising:
(a) collecting tissue capable of being propagated from a plant of lettuce cultivar Takita;
(b) cultivating the tissue to obtain proliferated shoots;
(c) rooting the proliferated shoots to obtain rooted plantlets; and
(d) optionally, growing plants from the rooted plantlets.

### Claim 18 (depends on 17)

A lettuce plantlet or plant obtained by the method of claim 17, wherein the lettuce plantlet or plant expresses all of the physiological and morphological characteristics of lettuce cultivar Takita.

### Claim 19 (depends on 2)

A method of introducing a desired added trait into lettuce cultivar Takita, the method comprising:
(a) crossing the plant of claim 2 with a lettuce plant that comprises a desired added trait to produce F1 progeny;
(b) selecting an F1 progeny that comprises the desired added trait;
(c) crossing the selected F1 progeny with lettuce cultivar Takita to produce backcross progeny;
(d) selecting a backcross progeny comprising the desired added trait; and
(e) optionally repeating steps (c) and (d) one or more times to produce a further backcross progeny plant derived from lettuce cultivar Takita comprising the desired added trait and otherwise all of the physiological and morphological characteristics of lettuce cultivar Takita, wherein in step (c) the selected backcross progeny produced in step (d) is used in place of the selected F1 progeny of step (b).

### Claim 20 (depends on 19)

The method of claim 19, wherein the desired added trait is male sterility, pest resistance, insect resistance, disease resistance, herbicide resistance, or any combination thereof.

### Claim 21 (depends on 19)

A lettuce plant produced by the method of claim 19 or a selfed progeny thereof, wherein the lettuce plant is the backcross progeny of step (d) or (e) or a selfed progeny thereof and has the desired added trait.

### Claim 22 (depends on 21)

A seed produced by crossing the plant of claim 21 with a second lettuce plant, wherein the seed produces a plant that has the desired added trait.

### Claim 23 (depends on 21)

A seed that produces the plant of claim 21.

### Claim 24 (depends on 2)

A method of producing a plant of lettuce cultivar Takita comprising a desired added trait, the method comprising introducing a transgene conferring the desired trait into the plant of claim 2.

### Claim 25 (depends on 24)

A lettuce plant produced by the method of claim 24 or a selfed progeny thereof, wherein the lettuce plant is a transformed plant of lettuce cultivar Takita and has the desired added trait.

### Claim 26 (depends on 25)

A seed of the plant of claim 25, wherein the seed produces a transformed plant that has the desired added trait.

### Claim 27 (depends on 2)

A method of determining a genotype of lettuce cultivar Takita, the method comprising:
(a) obtaining a sample of nucleic acids from the plant of claim 2; and
(b) detecting a polymorphism in the nucleic acid sample.

### Claim 28 (depends on 2)

A method of producing a lettuce leaf, the method comprising:
(a) growing the lettuce plant according to claim 2 to produce a lettuce leaf; and
(b) harvesting the lettuce leaf.

### Claim 29 (depends on 21)

A method of producing a lettuce leaf, the method comprising:
(a) growing the lettuce plant according to claim 21 to produce a lettuce leaf; and
(b) harvesting the lettuce leaf.