Linalool composition and method of producing therefor

A method for producing a composition comprising volatile components, wherein said volatile components comprise 60% or more linalool, and linalool consists of R-linalool, S-linalool, or both,

• the method comprising:
• A) culturing a microorganism expressing linalool synthase in a culture medium, and
• B) accumulating the composition in the culture medium,
• C) collecting the composition from the culture medium or the microorganism;
• wherein the microorganism has an ability to synthesize dimethylallyl diphosphate via a methylerythritol phosphate pathway and/or an ability to synthesize dimethylallyl diphosphate via a mevalonate pathway, and
• wherein the microorganism is a bacterium belonging to the genus Pantoea, and
• wherein a 2-ketogluconate formation pathway is blocked in the bacterium, and
• wherein said linalool synthase has a property selected from the group consisting of:
• (a) an amino acid sequence comprising at least one motif of the following formula:
• DDX1[F/Y][D/Y]X2X3G (SEQ ID No:
• 165), wherein D represents aspartic acid, F represents phenylalanine, Y represents tyrosine, G represents glycine, X1, X2, and X3 each independently represent an arbitrary amino acid, [F/Y] represents F or Y, and [D/Y] represents D or Y, and wherein the at least one motif is selected from the group consisting of:
• (i) a combination in which X1 is I, [F/Y] is F, [D/Y] is D, X2 is V, and X3 is Y,
• (ii) a combination in which X1 is I, [F/Y] is F, [D/Y] is D, X2 is V, and X3 is Y,
• (iii) a combination in which X1 is I, [F/Y] is F, [D/Y] is D, X2 is V, and X3 is H,
• (iv) a combination in which X1 is I, [F/Y] is F, [D/Y] is D, X2 is T, and X3 is Y,
• (v) a combination in which X1 is I, [F/Y] is F, [D/Y] is Y, X2 is V, and X3 is C,
• (vi) a combination in which X1 is I, [F/Y] is Y, [D/Y] is D, X2 is I, and X3 is Y,
• (vii) a combination in which X1 is I, [F/Y] is Y, [D/Y] is D, X2 is A, and X3 is Y,
• (viii) a combination in which X1 is I, [F/Y] is Y, [D/Y] is D, X2 is V, and X3 is Y,
• (ix) a combination in which X1 is V, [F/Y] is Y, [D/Y] is D, X2 is I, and X3 is Y,
• (x) a combination in which X1 is V, [F/Y] is Y, [D/Y] is D, X2 is V, and X3 is F,
• (xi) a combination in which X1 is M, [F/Y] is Y, [D/Y] is D, X2 is I, and X3 is Y, and
• (xii) a combination in which X1 is F, [F/Y] is F, [D/Y] is D, X2 is V, and X3 is E;
• (b) said linalool synthase is native to a microorganism belonging to the genus Streptomyces;
• and
• (c) combinations thereof.