Amadoriase having improved specific activity
AssigneeKikkoman Corporation
InventorsAtsushi Ichiyanagi, Airi Komatsuzaki
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Abstract
This invention provides an amadoriase having improved specific activity on a glycated substrate, compared with conventional amadoriase. Provided is an amadoriase comprising a substitution of the amino acid at the position corresponding to position 64 of the amino acid sequence as shown in SEQ ID NO: 1 with an amino acid selected from the group consisting of glycine, serine, methionine, leucine, threonine, valine, and isoleucine, a method for measurement of HbA1c, and a reagent kit for measurement of HbA1c using such amadoriase. Such method and kit for measurement enable rapid, simple, and accurate quantification of HbA1c.
Claims14
An amadoriase selected from the group consisting of the following (i) to
- (i) an amadoriase comprising an amino acid sequence having 90% or higher sequence identity with the amino acid sequence as shown in SEQ ID NO:
- 1 over the full length, wherein the amino acid at the position corresponding to position 64 of the amino acid sequence as shown in SEQ ID NO:
- 1 is an amino acid selected from the group consisting of glycine, methionine, leucine, threonine, valine, and isoleucine and having amadoriase activity on a glycated peptide;
- and
- (ii) an amadoriase comprising an amino acid sequence having 90% or higher sequence identity with the amino acid sequence as shown in SEQ ID NO:
- 1 over the full length, wherein the amino acid at the position corresponding to position 64 of the amino acid sequence as shown in SEQ ID NO:
- 1 is an amino acid selected from the group consisting of glycine, methionine, leucine, threonine, valine, and isoleucine and having amadoriase activity on α-fructosyl-valyl-histidyl-leucyl-threonyl-prolyl-glutamyl-glutamyl-lysine (αF8P).
An amadoriase comprising the amino acid sequence as shown in SEQ ID NO:
- 211 or 213.
Dependencies are inferred from the claim text.