Chimeric antigen receptors based on single domain antibodies and methods of use thereof
AssigneeLegend Biotech USA Inc.
InventorsXiaohu Fan, Chuan-Chu Chou, Qiuchuan Zhuang, Pingyan Wang, Lin Wang, Lei Yang, Jiaying Hao
Life of this patent
Estimating the term…
Abstract
The present application provides single-domain antibodies, and chimeric antigen receptors comprising one or more antigen binding domains each comprising a single-domain antibody. Further provided are engineered immune effector cells (such as T cells) comprising the chimeric antigen receptors. Pharmaceutical compositions, kits and methods of treating cancer are also provided.
Claims24
A chimeric antigen receptor (CAR) comprising a polypeptide comprising,
- (a) an extracellular antigen binding domain comprising a first single domain antibody (sdAb) specifically binding to a first epitope of BCMA and a second sdAb specifically binding to a second epitope of BCMA, wherein each of the first and second sdAb is a VHH domain, and wherein the first sdAb comprises the amino acid sequence of SEQ ID NO:
- 87 and the second sdAb comprises the amino acid sequence of SEQ ID NO:
- 80;
- (b) a transmembrane domain;
- and
- (c) an intracellular signaling domain.
The CAR of 1, wherein the intracellular signaling domain comprises a primary intracellular signaling domain of an immune effector cell.
Dependencies are inferred from the claim text.