Methods and compositions for improving plant traits
AssigneePivot Bio, Inc.
InventorsKarsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung
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Abstract
Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.
Claims29
An engineered, non-intergeneric diazotroph having increased nitrogen fixation activity as compared to an unmodified organism of the same species as said engineered, non-intergeneric diazotroph, wherein said engineered, non-intergeneric diazotroph comprises a genetic variation within one or more genes for regulating nitrogen fixation or assimilation, said genetic variation comprising genetic material that originates from at least one organism of the same species as said engineered, non-intergeneric diazotroph.
Dependencies are inferred from the claim text.