Compositions comprising digestive enzymes
AssigneeClara Foods Co.
InventorsHalle Redfearn, Harshal Kshirsagar, Kritika Mahadevan, Alexandre Chapeaux, Wesley Rutherford-Jenkins
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Abstract
Provided herein are compositions with enhanced protein specific activity, protein combinations and methods for the preparation thereof.
Claims20
A composition comprising a recombinant pepsin polypeptide, wherein the composition is (a) free from animal-derived proteins, (b) the pepsin polypeptide is in a proteolytically inactive form which does not self-digest, (c) the composition has a pH greater than 5.4, and (d) the composition has a specific activity of at least 30,000 FCC units/mg total protein;
- wherein the composition is in powdered form.
A method of producing a high-activity stable pepsin powdered composition comprising:
- a. providing a microorganism expressing a recombinant pepsinogen, wherein the expressed pepsinogen is secreted by the microorganism into the growth media;
- b. harvesting the growth media and removing the cells therefrom to obtain a liquid starting material;
- c. lowering the pH of the liquid starting material to less than pH 4.0 to obtain an activated pepsin composition;
- d. raising the activated pepsin composition to a pH of greater than 5.4 to obtain a high-activity stable pepsin composition having a specific activity of at least 10,000 FCC units/mg total powder;
- and
- e. isolating the stable pepsin polypeptide from protein and small molecules in the liquid starting material to obtain a powdered composition;
- wherein step (e) is performed after steps (c) and (d).
Dependencies are inferred from the claim text.