Polysaccharide-polyamine copolymer and use thereof in reducing low density lipolipoprotein (LDL) concentration in plasma

A method for the treatment of hypercholesterolemia, comprising:

• administering a pharmaceutical composition comprising an amino-polysaccharide copolymer or a pharmaceutically acceptable salt thereof as an active ingredient, wherein, the amino-polysaccharide copolymer is a copolymerization product of the following two parts:
• a selectively oxidized polysaccharide having a 2,3-dialdehyde moiety, and an amino polymer which provides an amino functionality, wherein the selectively oxidized polysaccharide is one or more selected from the group consisting of selectively oxidized cellulose, selectively oxidized amylopectin, and selectively oxidized chitosan and wherein the amino polymer is at least one selected from the group consisting of polyethyleneimine (PEI) and poly(allylamine) (PLA), and
• the amino polymer cross linking the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety to form a net-like structure and finally to provide a hydrogel having an amino functionality and a particulate amino-polysaccharide copolymer, wherein a carbon nitrogen double bond resulted from the crosslinking is reduced to a single bond;
• amino functionalities of the hydrogel having an amino functionality and the particulate amino-polysaccharide copolymer can be protonated to form a cationic copolymer having three dimensional network structure and protonation sites,
• wherein a nitrogen content of the cationic copolymer or the amino-polysaccharide copolymer is at least 12.3% weight percent, or at least 15% weight percent, or at least 22% weight percent, or at least 40% weight percent, and the cationic copolymer and the amino-polysaccharide copolymer are both insoluble in water, and
• wherein in the selectively oxidized polysaccharide having a 2,3-dialdehyde moiety, a ratio of an amount of oxidized glucose units to the total amount of glucose units is at least 50%, or at least 70%, or at least 80%.