Methods of producing lipids
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsJames Robertson Petrie, Thomas Vanhercke, Pushkar Shrestha, Qing Liu, Surinder Pal Singh
Life of this patent
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Abstract
The present invention relates to methods of producing lipids. In particular, the present invention relates to methods of increasing the level of one or more non-polar lipids and/or the total non-polar lipid content in a transgenic organism or part thereof. In one particular embodiment, the present invention relates to the use of an acyltransferase, for example, a monoacylglycerol acyltransferase (MGAT) to increase the level of one or more non-polar lipids and/or the total non-polar lipid content in plants, plant seed and/or leaves, algae and fungi.
Claims16
A plant comprising
- i) a first exogenous polynucleotide which encodes a monoacylglycerol acyltransferase (MGAT) and which is expressed in a vegetative part of the plant or, if the plant is a unicellular plant, in the unicellular plant,
- ii) a second exogenous polynucleotide which encodes a diacylglycerol acyltransferase (DGAT), a glycerol-3-phosphate acyltransferase (GPAT), or both, and which is expressed in a vegetative part of the plant or, if the plant is a unicellular plant, in the unicellular plant, and
- iii) a level of triacylglycerol (TAG) in the vegetative part of the plant or in the unicellular plant that is greater on a relative basis when compared to such a vegetative part of a plant or unicellular plant, respectively, lacking the first exogenous polynucleotide and comprising the second exogenous polynucleotide.
A plant comprising:
- i) a first exogenous polynucleotide which encodes a monoacylglycerol acyltransferase (MGAT) and which is expressed in a vegetative part of the plant or, if the plant is a unicellular plant, in the unicellular plant,
- ii) a second exogenous polynucleotide which encodes a diacylglycerol acyltransferase (DGAT), a glycerol-3-phosphate acyltransferase (GPAT), or both, and which is expressed in a vegetative part of the plant or, if the plant is a unicellular plant, in the unicellular plant, and
- iii) a level of triacylglycerol (TAG) in the vegetative part of the plant or in the unicellular plant that is at least 30% greater on a relative basis when compared to such a vegetative part of the plant or unicellular plant, respectively, lacking the first exogenous polynucleotide and comprising the second exogenous polynucleotide.
A plant comprising
- i) a first exogenous polynucleotide which encodes a monoacylglycerol acyltransferase (MGAT) and which is expressed in a vegetative part of the plant or, if the plant is a unicellular plant, in the unicellular plant,
- ii) a second exogenous polynucleotide which encodes a diacylglycerol acyltransferase (DGAT), a glycerol-3-phosphate acyltransferase (GPAT), or both, and which is expressed in a vegetative part of the plant or, if the plant is a unicellular plant, in the unicellular plant, and
- iii) a level of triacylglycerol (TAG) in the vegetative part of the plant or in the unicellular plant that is at least 90% greater on a relative basis when compared to such a vegetative part of the plant or unicellular plant, respectively, lacking the first exogenous polynucleotide and comprising the second exogenous polynucleotide.
Dependencies are inferred from the claim text.