Probes for quantitative imaging of thiols in various environments

A method of detecting a thiol in an environment, said method comprising:

• exposing the environment to a probe molecule,
• wherein the probe molecule comprises the following structure:
• wherein the probe molecule reversibly reacts with the thiol in the environment to form a probe-thiol adduct, thereby causing a ratiometric change in a spectrometric property of the probe molecule and the probe-thiol adduct;
• and
• correlating the ratiometric change in the spectrometric property of the probe molecule and the probe-thiol adduct to presence of the thiol in the environment, wherein the correlating comprises quantifying the concentration of the thiol in the environment.

A method of detecting a thiol in an environment, said method comprising:

• exposing the environment to a probe molecule,
• wherein the probe molecule comprises the following structure:
• wherein the probe molecule reversibly reacts with the thiol in the environment to form a probe-thiol adduct, thereby causing a ratiometric change in a spectrometric property of the probe molecule and the probe-thiol adduct;
• and
• correlating the ratiometric change in the spectrometric property of the probe molecule and the probe-thiol adduct to presence of the thiol in the environment, wherein the correlating comprises quantifying the concentration of the thiol in the environment.

A method of detecting a thiol in an environment, said method comprising:

• exposing the environment to a probe molecule, wherein the probe molecule comprises the following structure:
• wherein the probe molecule reversibly reacts with the thiol in the environment to form a probe-thiol adduct, thereby causing a ratiometric change in a spectrometric property of the probe molecule and the probe-thiol adduct;
• and
• correlating the ratiometric change in the spectrometric property of the probe molecule and the probe-thiol adduct to presence of the thiol in the environment, wherein the correlating comprises quantifying the concentration of the thiol in the environment.

A method of detecting a thiol in an environment, said method comprising:

• exposing the environment to a probe molecule, wherein the probe molecule is selected from the group consisting of:
• wherein AM comprises —CH2OCOCH3 and R comprises one of
• wherein the probe molecule reversibly reacts with the thiol in the environment to form a probe-thiol adduct, thereby causing a ratiometric change in a spectrometric property of the probe molecule and the probe-thiol adduct;
• and
• correlating the ratiometric change in the spectrometric property of the probe molecule and the probe-thiol adduct to presence of the thiol in the environment, wherein the correlating comprises quantifying the concentration of the thiol in the environment.