Methods and materials for producing 5 and 7-carbon monomers
AssigneeINV Nylon Chemicals Americas, LLC
InventorsAlex Van Eck Conradie, Adriana Leonora Botes
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Abstract
This document describes biochemical pathways for biosynthesizing a 3-oxo-7-hydroxyheptanoyl-CoA intermediate using a β-ketothiolase, and enzymatically converting 3-oxo-7-hydroxyheptanoyl-CoA to 7-hydroxyheptanoic acid. —7-hydroxyheptanoic acid can be further enzymatically converted to pimelic acid, 7-aminoheptanoic acid, heptamethylenediamine or 1,7-heptanediol. This document also describes recombinant hosts producing 7-hydroxyheptanoic acid as well as pimelic acid, 7-aminoheptanoic acid, heptamethylenediamine and 1,7-heptanediol.
Claims27
A method of producing 3-oxo-7-hydroxyheptanoyl-CoA or a salt thereof, said method comprising enzymatically converting 5-hydroxypentanoyl-CoA to 3-oxo-7-hydroxyheptanoyl-CoA using a polypeptide having the activity of a β-ketothiolase classified under EC 2.3.1.16 or EC 2.3.1.174.
Dependencies are inferred from the claim text.