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US 10,920,251 B2Granted
Microbial production of fats
AssigneeWilliam Marsh Rice University
InventorsKa-Yiu San, Zhilin Li, Xian Zhang
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Abstract
This invention describes a method of using microbial to produce fats, such as fatty acids and their derivatives, or products derived from the fatty acid synthesis cycle, such as hydroxyfatty acids, methyl ketones, and the like.
Claims9
A method of producing products in bacteria, comprising:
- a) aerobically culturing a bacteria in a growth medium until sufficient cell mass is reached;
- b) smoothly transitioning from aerobic culturing to culturing under oxygen lean conditions (>0.1% dissolved oxygen (“DO”) to <5% DO) over a course of time of 1-12 hrs;
- c) further culturing said bacteria under oxygen lean conditions by only sparging a head space with O2 containing gas until product is formed;
- and
- d) isolating said product from said bacteria, said growth medium, or both.
2 more elements
Dependencies are inferred from the claim text.