Winter aconite fatty acid elongase and uses thereof in the production of fatty acids
AssigneeNational Research Council of Canada
InventorsDauenpen Meesapyodsuk, Xiao Qiu, Bob Chapman
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Abstract
Fatty acids, and methods for the production thereof, are provided. Transgenic organisms, microbes, plants, seeds, and cells useful in the production of fatty acids, along with related expression vectors, phages, plasmids, nucleic acids, and enzymes, are also provided. Methods for the production of fatty acids such as docosadienoic acid and docosatrienoic acid, involving the use of winter aconite (Eranthis hyemalis) EhELO1 elongase, are described in detail.
Claims20
An isolated nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having elongase activity, wherein said polypeptide has at least 95% sequence identity to the full length of the amino acid sequence set forth in SEQ ID NO:
- 4, and wherein the nucleotide sequence comprises at least one nucleotide substitution, insertion, or non-terminal deletion relative to the nucleotide sequence set forth in SEQ ID NO:
- 5.
An expression vector, phage, or plasmid comprising a nucleic acid molecule, said nucleic acid molecule comprising:
- i) the nucleotide sequence set forth in SEQ ID NO:
- 5;
- ii) the nucleotide sequence set forth in SEQ ID NO:
- 6;
- or
- iii) a nucleotide sequence encoding a polypeptide having elongase activity, wherein said polypeptide has at least 95% sequence identity to the full length of the amino acid sequence set forth in SEQ ID NO:
- 4,
- wherein said nucleic acid molecule is operably linked to a heterologous regulatory element.
An ex vivo method for producing at least one unsaturated fatty acid comprising 20 or more carbons from a substrate feedstock, the method comprising:
- a) providing the substrate feedstock;
- and
- b) exposing the substrate feedstock to a polypeptide having elongase activity, wherein said polypeptide has at least 95% sequence identity to the full length of the amino acid sequence set forth in SEQ ID NO:
- 4, wherein the substrate feedstock comprises 18:1-9 fatty acid;
- 18:1-11 fatty acid;
- 20:1-11 fatty acid;
- 20:1-13 fatty acid;
- 22:1-15 fatty acid;
- 24:1-17 fatty acid;
- 18:2-9,12 fatty acid;
- 20:2-11,14 fatty acid;
- 22:2-13,16 fatty acid;
- 18:3-9,12,15 fatty acid;
- 20:3-11,14,17 fatty acid;
- 22:3-13,16,19 fatty acid;
- 18:3-6,9,12 fatty acid;
- 20:3-8,11,14 fatty acid;
- 18:4-6,9,12,15 fatty acid;
- 20:4-8,11,14,17 fatty acid;
- 22:4-10,13,16,19 fatty acid;
- 20:4-5,8,11,14 fatty acid;
- 22:4-7,10,13,16 fatty acid;
- 20:5-5,8,11,14,17 fatty acid;
- 22:5-7,10,13,16,19 fatty acid;
- 22:6-4,7,10,13,16,19 fatty acid;
- or a combination of two or more thereof.
Dependencies are inferred from the claim text.