Back to results
US 10,920,189 B2Granted
Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli
AssigneeInscripta, Inc.
InventorsRichard Fox, Daniel Held, Eric Abbate, Michael Clay, Katherine Krouse, Nandini Krishnamurthy, Krishna Yerramsetty
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.
Claims20
An engineered E. coil cell comprising the following variant sequences:
- a dapA protein having the amino add sequence of SEQ ID No. 1 and a dapA gene promoter sequence having the nucleic add sequence of SEQ ID No. 2 driving transcription of the dapA protein;
- and further comprising an additional variant protein selected from the following variant proteins:
- a lysC protein haying the amino add sequence of SEQ ID No. 3, a garb protein encoded by the nucleic add sequence of SEQ. ID No. 4, a yicL protein encoded by the nucleic add sequence of SEQ ID No. 5, a lysP protein having the amino add sequence of SEQ ID No. 6, a mgSA protein encoded by the nucleic add sequence of SEQ ID No. 7, and a pckE protein having the amino add sequence of SEQ ID No. 8.
An engineered E. coil cell comprising the following variant sequences:
- a promoter sequence having the nucleic add SEQ ID No. 2 driving transcription of a dapA gene, and further comprising one of the following proteins:
- an amyA protein encoded by the nucleic add sequence of SEQ ID No. 9, an amyA protein having the amino add sequence of SEQ ID No. 10, a cysN protein having the amino add sequence of SEQ ID No. 11, a dosP protein encoded by the nucleic add sequence of SEQ ID No. 12, a emrE protein encoded by the nucleic add sequence SEQ ID No. 13, a focB protein encoded by the nucleic add sequence SEQ ID No. 14, a glnD protein encoded by the nucleic add sequence SEQ. ID No. 15, a glnE protein having the amino acid sequence of SEQ ID No. 16, a hicB protein encoded by the nucleic add sequence of SEQ No. 17, a macB protein encoded by the nucleic acid sequence of SEQ ID No. 18, a marA protein having the amino add sequence of SEQ ID No. 19, or a metL protein having the amino add sequence of SEQ ID No. 20.
Dependencies are inferred from the claim text.