Methods and systems for identification of bacteria in biological fluid using raman spectroscopy and applications of same

A system for identification and discrimination of bacteria and/or mutant bacterial strains in a biological fluid, comprising:

• an optical probe optically connected to a light source for emitting a beam of light and configured to deliver the beam of light emitted from the light source to the biological fluid and to collect light scattered from the biological fluid;
• a detector optically coupled with the optical probe, for obtaining Raman spectra from the collected scattered light;
• and
• a controller in communication with the detector and programmed for finding Raman signatures corresponding to each type of bacteria and/or mutant and/or resistant bacterial strains from the obtained Raman spectra and identifying and discriminating each type of bacteria and/or mutant and/or resistant bacterial strains in the biological fluid from the Raman signatures,
• wherein said finding the Raman signatures comprises processing the obtained Raman spectra with a multiclass support vector machine (SVM) algorithm and a linear discriminant analysis to find the Raman signatures corresponding to each type of bacteria and/or mutant and/or resistant bacterial strains.

A system for characterizing contents of a bio-object, comprising:

• an optical probe optically connected to a light source for emitting a beam of light and configured to deliver the beam of light emitted from the light source to the bio-object and to collect light scattered from the bio-object;
• a detector optically coupled with the optical probe, for obtaining Raman data from the collected scattered light;
• and
• a controller in communication with the detector and programmed for finding Raman signatures corresponding to biochemical compositions from the obtained Raman data and identifying and discriminating the contents of the bio-object from the Raman signatures,
• wherein the optical probe comprises a plurality of optical fibers spatially arranged in a fiber array;
• wherein at least one fiber of the plurality of optical fibers is a source optical fiber for delivering the beam of light emitted from the light source to the surface of the bio-object, and the remaining fibers of the plurality of optical fibers are collection optical fibers for collecting light scattered from the illuminated bio-object;
• and
• wherein the optical probe further comprises at least one light-emitting diode (LED) for determining placement information of the optical probe.