Spatial mapping of nucleic acid sequence information

A method, comprising:

• (a) providing a capture array comprising a plurality of capture sites, wherein each capture site of the plurality of capture sites comprises:
• a pair of probes that are separately immobilized on a surface at each capture site, wherein a first probe of the pair of probes comprises a first primer binding region sequence and a spatial address region sequence and does not comprise a poly-T capture sequence, wherein the spatial address region sequence of the first probe comprises a sequence of nucleic acids unique to each capture site such that the spatial address region sequence of each capture site of the plurality of capture sites is different relative to one another, wherein a second probe of the pair of probes comprises a second primer binding region sequence and the poly-T capture sequence and does not comprise the spatial address region sequence and wherein the second probe is a same sequence at each of the plurality of capture sites, wherein the poly-T capture sequence is configured to hybridize to a target nucleic acid comprising a poly-A tail;
• (b) contacting the plurality of capture sites of the capture array with a tissue sample such that a position of each capture site on the capture array can be correlated with a position in the tissue sample;
• (c) subsequent to the contacting, allowing target nucleic acids of the tissue sample, the target nucleic acids comprising the poly-A tail, to hybridize to respective poly-T capture sequences of the second probe of the plurality of capture sites to form hybridized second probes;
• (d) extending the poly-T capture region sequence of the hybridized second probes to form first complementary strands of the sample nucleic acids;
• and
• (e) linking ends of the first complementary strands to the first probes at each capture site to form immobilized first complementary strands and such that the immobilized first complementary strand comprises the unique spatial address region sequence of the first capture probe associated with each respective capture site and such that immobilized first complementary strands at different capture sites comprise different spatial address region sequences.