Yeast strains for ethanol production
AssigneeDsm IP Assets B.V.
InventorsIoannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk
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Abstract
This invention relates to a recombinant cell, preferably a recombinant yeast cell comprising: a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP+ and/or for NADPH; b) a gene encoding an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2. Said cell is suitable for ethanol production, has a reduced glycerol production at high ethanol yield.
Claims15
A recombinant cell, optionally a recombinant yeast cell comprising:
- a) a gene encoding an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP and/or for NADPH;
- b) a gene encoding an enzyme having at least NAD dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10);
- and
- c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2.
Dependencies are inferred from the claim text.