Method for measuring activity of oxidoreductase
AssigneeNational Agriculture and Food Research Organization
InventorsToshimasa Yamazaki, Yuki Nishigaya
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Abstract
The present invention relates to a method for measuring activity of HAO which comprises bringing HAO into contact with hydroxylamine in the presence of a tetrazolium salt. Further, the present invention also provides a method for culturing microorganisms which comprises culturing microorganisms in a storage container of a sample containing microorganisms installed separately in a liquid medium storage container, wherein the storage container of a sample containing microorganisms has pores.
Claims5
A method for measuring activity of hydroxylamine oxidoreductase (HAO), the method comprising bringing a sample comprising HAO into contact with hydroxylamine in the presence of a resazurin salt, wherein the hydroxylamine is contacted with at a molar ratio of at least ten times of the resazurin salt, and the measurement is carried out at pH of 4.6 to 7.6, and measuring the activity of HAO in the sample by fluorescence measurement.
Dependencies are inferred from the claim text.