Production of viruses in avian eggs
AssigneesCommonwealth Scientific and Industrial Research Organisation, University of Georgia Research Foundation, Inc.
InventorsAndrew Bean, John William Lowenthal, Luis Fernando Malaver-Ortega, Ralph A. Tripp
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Abstract
The present invention relates to modified avian eggs which can be used to produce increased levels of virus. The present invention also relates to methods of producing viruses in avian eggs of the invention, as well as the use of the viruses obtained to prepare vaccine compositions.
Claims26
An avian egg comprising a genetic modification which reduces the expression of an antiviral gene in the egg when compared to an isogenic egg lacking the genetic modification, wherein the antiviral gene is selected from IL-1RA, IL-6, CNOT4, BACE2, UBA5, ZFPM2, TRIM50, DDI2, NPR2, CAPN13, DNASE1L2, PHF21A, PCGF5, LAMP1, EFR3A, ABI1, GADL1, PLVAP, CYYR1, ASAP1, NXF1, NSUN6, ANGPTL7, SIL1, BCAR3, GOLPH3L, HN1, ADCY7, CBLN4, CXORF56, DDX10, EIF2S3, ESF1, GCOM1, GTPBP4, IFT43, KPNA3, LRRIQ1, LUC7L, MRPL12, PWP2, RPL7A, SMYD2, XPO1 and ZKSCAN7.
A transgenic avian comprising a genetic modification, wherein the genetic modification reduces expression of an antiviral gene in an egg produced by the avian compared to an isogenic egg produced by an isogenic avian lacking the genetic modification, and wherein the antiviral gene is selected from IL-1RA, IL-6, CNOT4, BACE2, UBA5, ZFPM2, TRIM50, DDI2, NPR2, CAPN13, DNASE1L2, PHF21A, PCGF5, LAMP1, EFR3A, ABI1, GADL1, PLVAP, CYYR1, ASAP1, NXF1, NSUN6, ANGPTL7, SIL1, BCAR3, GOLPH3L, HN1, ADCY7, CBLN4, CXORF56, DDX10, EIF2S3, ESF1, GCOM1, GTPBP4, IFT43, KPNA3, LRRIQ1, LUC7L, MRPL12, PWP2, RPL7A, SMYD2, XPO1 and ZKSCAN7.
Dependencies are inferred from the claim text.