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US 10,906,984 B2Granted
CAR expression vector and CAR-expressing T cells
AssigneeYamaguchi University
InventorsKoji Tamada, Yukimi Sakoda, Keishi Adachi
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AssigneeYamaguchi University
InventorsKoji Tamada, Yukimi Sakoda, Keishi Adachi
Estimating the term…
An object of the present invention is to provide CAR-expressing T cells that coexpress a chimeric antigen receptor (CAR) and a T cell immune function-enhancing factor and have a high immunity-inducing effect and antitumor activity, and to provide a CAR expression vector for the preparation of the CAR-expressing T cells. A CAR expression vector comprises a nucleic acid encoding a chimeric antigen receptor (CAR) and a nucleic acid encoding a T cell immune function-enhancing factor, wherein the nucleic acid encoding an immune function-enhancing factor is a nucleic acid encoding interleukin-7 and a nucleic acid encoding CCL19, a nucleic acid encoding a dominant negative mutant of SHP-1, or a nucleic acid encoding a dominant negative mutant of SHP-2, or a CAR-expressing T cell introduced with the CAR expression vector are prepared.
A CAR expression vector comprising a nucleic acid encoding a chimeric antigen receptor (CAR), a nucleic acid encoding interleukin-7, and a nucleic acid encoding CCL19, wherein interleukin-7 is the only interleukin encoded by the CAR expression vector.
An isolated CAR-expressing T cell, or a culture comprising the isolated CAR-expressing T cell, said isolated T cell prepared by introducing a first CAR expression vector that comprises a nucleic acid encoding a CAR and only one nucleic acid encoding an interleukin, wherein the interleukin consists of interleukin-7, and a second CAR expression vector that comprises a nucleic acid encoding a CAR and a nucleic acid encoding CCL19, wherein interleukin-7 is the only type of interleukin encoded by a nucleic acid introduced into the isolated T cell;
An isolated T cell expressing a CAR, interleukin-7, and CCL19, or a culture comprising the isolated T cell, said isolated T cell comprising one or more recombinant constructs, wherein at least one recombinant construct encodes a CAR, at least one recombinant construct encodes interleukin-7 and at least one recombinant construct encodes CCL19, wherein interleukin-7 is the only type of interleukin encoded by any recombinant construct.
A method for producing a T cell expressing a CAR, interleukin-7, and CCL19, comprising introducing a recombinant construct encoding the CAR, a recombinant construct encoding interleukin-7 and a recombinant construct encoding CCL19 into the T cell using a vector, wherein interleukin-7 is the only type of interleukin encoded by any recombinant construct that is introduced into the T-cell.
An isolated T cell expressing a CAR from a recombinant construct encoding a CAR, interleukin-7 from a recombinant construct encoding interleukin-7, and CCL19 from a recombinant construct encoding CCL19, wherein interleukin-7 is the only interleukin expressed from any recombinant construct within the isolated T cell.
Dependencies are inferred from the claim text.