Microscope system

A microscope system for observing a specimen via an objective lens that forms an observation image of the specimen, the microscope system comprising:

• a stage on which the specimen is mounted;
• a plurality of light sources configured to emit light irradiating the specimen, wherein the light sources include a DF (Dark Field) ring light source and a coaxial light source;
• an illumination optical system configured to irradiate the specimen with the light emitted from the light sources;
• an operating unit configured to receive selection of the light sources and setting of at least one of a state and an amount of light of each of the light sources;
• a focusing unit configured to move at least one of the stage and the objective lens in a direction orthogonal to a mounting surface on which the specimen is mounted to adjust a distance between the stage and the objective lens;
• an imaging unit configured to generate a plurality of pieces of image data by imaging the observation image of the specimen formed by the objective lens a plurality of times, wherein a lighting segment that emits light from among a plurality of segments of the DF ring light source is changed each time the imaging unit images the observation image to generate one of the pieces of image data;
• and
• a combined image generating unit configured to combine the plurality of pieces of image data generated by the imaging unit to generate combined image data,
• wherein:
• the microscope system enables selection of a state of optical elements constituting the illumination optical system and selection of a type, the state, and an amount of light of the light sources, in generating the plurality of pieces of image data that are combined by the combined image generating unit to generate the combined image data, and
• the combined image generating unit is an EFI (Extended Focus Imaging) generating unit configured to generate extended focus image data as the combined image data.