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US 10,895,728 B2Granted
Observation method and holder for gel-like transparent sample which encloses an observation target
AssigneeOlympus Corporation
InventorsYoshihiro Shimada, Kazuhito Goda
Life of this patent
Estimating the term…
Abstract
Provided is a sample observation method including: bringing a gel-like transparent sample that encloses an observation target into contact with a transparent flat surface section of a substrate; and collecting light from the observation target by means of an objective lens via the substrate, in a state in which a pressing force is made to act in a direction in which the sample and the flat surface section relatively approach each other, until the contact area between the sample and the flat surface section comes to have a size allowing an effective light flux for the objective lens of a microscope to pass therethrough.
Claims23
A sample observation method comprising:
- bringing a gel-like transparent sample that encloses an observation target into contact with a transparent flat surface section of a substrate;
- and
- collecting light from the observation target by means of an objective lens of a microscope via the substrate, in a state in which a pressing force is made to act in a direction in which the sample and the flat surface section relatively approach each other, until a contact area between the sample and the flat surface section comes to have a size allowing an effective light flux for the objective lens to pass therethrough,
- wherein the pressing force is made to occur due to gravity.
1 more element
A sample observation method comprising:
- bringing a gel-like transparent sample that encloses an observation target into contact with an objective lens of a microscope;
- and
- collecting light from the observation target by means of the objective lens, in a state in which a pressing force is made to act in a direction in which the sample and the objective lens relatively approach each other, until a contact area between the sample and the objective lens comes to have a size allowing an effective light flux for the objective lens to pass therethrough,
- wherein the pressing force is made to occur due to gravity.
1 more element
A sample observation method comprising:
- bringing a gel-like transparent sample that encloses an observation target into contact with a transparent flat surface section of a substrate;
- and
- collecting light from the observation target by means of an objective lens of a microscope via the substrate, in a state in which a pressing force is made to act in a direction in which the sample and the flat surface section relatively approach each other, until a contact area between the sample and the flat surface section comes to have a size allowing an effective light flux for the objective lens to pass therethrough,
- wherein the sample is dropped on the substrate, thus being brought into contact with the flat surface section.
1 more element
A sample observation method comprising:
- bringing a gel-like transparent sample that encloses an observation target into contact with a transparent flat surface section of a substrate;
- and
- collecting light from the observation target by means of an objective lens of a microscope via the substrate, in a state in which a pressing force is made to act in a direction in which the sample and the flat surface section relatively approach each other, until a contact area between the sample and the flat surface section comes to have a size allowing an effective light flux for the objective lens to pass therethrough,
- wherein the substrate has a form capable of accumulating a liquid therein;
- and
- wherein the sample and the flat surface section are brought into contact with each other in a state in which at least one section of the sample is immersed in liquid accumulated in the substrate.
3 more elements
A sample observation method comprising:
- bringing a gel-like transparent sample that encloses an observation target into contact with a transparent flat surface section of a substrate;
- and
- collecting light from the observation target by means of an objective lens of a microscope via the substrate, in a state in which a pressing force is made to act in a direction in which the sample and the flat surface section relatively approach each other, until a contact area between the sample and the flat surface section comes to have a size allowing an effective light flux for the objective lens to pass therethrough,
- wherein the sample is formed by causing a hanging drop to gel, the hanging drop being a transparent droplet, in which the observation target is enclosed, in a state in which the droplet is hung.
1 more element
A sample observation method comprising:
- bringing a gel-like transparent sample that encloses an observation target into contact with an objective lens of a microscope;
- and
- collecting light from the observation target by means of the objective lens, in a state in which a pressing force is made to act in a direction in which the sample and the objective lens relatively approach each other, until a contact area between the sample and the objective lens comes to have a size allowing an effective light flux for the objective lens to pass therethrough,
- wherein the sample is formed by causing a hanging drop to gel, the hanging drop being a transparent droplet, in which the observation target is enclosed, in a state in which the droplet is hung.
1 more element
A sample holder comprising:
- a substrate that has a transparent flat surface section with which a gel-like transparent sample that encloses at least one observation target is brought into contact;
- a contact-state maintaining unit that maintains a contact state in which a contact area between the sample and the flat surface section has a size allowing an effective light flux for an objective lens of a microscope to pass therethrough;
- and
- a base material that supports the sample in a state in which the sample is brought into contact with the flat surface section,
- wherein the base material can support a droplet in a hung state, with the droplet enclosing the observation target;
- and
- the sample is formed by causing the droplet, which is supported by the base material, to gel.
4 more elements
A sample holder comprising:
- a substrate that has a transparent flat surface section with which a gel-like transparent sample that encloses at least one observation target is brought into contact;
- a contact-state maintaining unit that maintains a contact state in which a contact area between the sample and the flat surface section has a size allowing an effective light flux for an objective lens of a microscope to pass therethrough;
- and
- a base material that supports the sample in a state in which the sample is brought into contact with the flat surface section,
- wherein the substrate has a form capable of accumulating a liquid therein and capable of immersing at least one section of the sample in liquid accumulated in the substrate.
2 more elements
Dependencies are inferred from the claim text.