Genetic alterations in isocitrate dehydrogenase and other genes in malignant glioma

A method of detecting an isocitrate dehydrogenase 1 (IDH1) mutation or an isocitrate dehydrogenase 2 (IDH2) mutation in a nucleic acid present in a tumor sample obtained from a human subject, wherein the tumor is a glioblastoma multiforme (GBM), comprising:

• sequencing the nucleic acid wherein the nucleic acid comprises the IDH1 mutation or the IDH2 mutation, wherein the IDH1 mutation is present in a codon that encodes amino acid 132 in the wild type IDH1 polypeptide of SEQ ID NO:
• 130;
• or
• wherein the IDH2 mutation is present in a codon that encodes amino acid 172 in the wild type IDH2 polypeptide of SEQ ID NO:
• 131;
• and wherein when the mutation is present, administering to the subject a treatment selected from the group consisting of:
• chemotherapy, radiation resection of the tumor, and combinations thereof.