Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes

A method of single cell whole genome amplification and sequencing comprising

• contacting double stranded genomic DNA from a single cell with Tn5 transposases each complexed with a transposon DNA, wherein the transposon DNA includes a double-stranded 19 bp Tnp binding site and an overhang, wherein the overhang includes a barcode region, a priming site and a strong T7 promoter sequence, wherein the Tn5 transposase/transposon DNA complex bind to target locations along the double stranded genomic DNA cleaving the double stranded genomic DNA into a plurality of double stranded fragments, with each double stranded fragment having a first complex attached to an upper strand by the Tnp binding site and a second complex attached to a lower strand by the Tnp binding site,
• removing the Tn5 transposases from the complex,
• extending the double stranded fragments along the transposon DNA to make a double stranded extension product having T7 promoters at each end,
• contacting the double stranded extension product with T7 RNA polymerase to make RNA transcripts of the double stranded extension product,
• reverse transcribing the RNA transcript into single stranded DNA, and
• forming a complementary strand to the single stranded DNA to form a double stranded DNA including the genomic DNA sequence and having barcodes at both ends of the upper and lower strands.